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KMID : 0361420020260040426
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Journal of Korean Academy of Rehabilitation Medicine
2002 Volume.26 No. 4 p.426 ~ p.431
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Effects on Myoblast Proliferation by Fibroblast Growth Factor, Leukemia Inhibitory Factor and Interleukin-1
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박윤길/Yoon Ghil Park
문재호/박은숙/김진/송남규/Jae Ho Moon/Eun Sook Park/Jin Kim/Nam Kyu Song
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Abstract
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Objective: Recently, cultured myoblast transplantation has been extensively studied as a gene complementation approach in such genetic diseases as Duchenne muscular dystrophy (DMD). In the present work we investigated the stimulating effects
of
the growth factors, such as basic fibroblast growth factor (bFGF), leukemia inhibitory factor (LIF) and interleukin-1 (IL-1), on growth rate and differentiation of myoblast.
Method: Human myoblasts were cultured from biopsy and treated in vitro with various concentration of bFGF, LIF and IL-1. In serum-free defined medium the following observation were made to evaluate differentiation.
Results: bFGF and LIF except IL-1 were found to have stimulating effect of myoblast proliferation comparing to control group (p＜0.05), yet there were no statistically significant differences among each growth factors (p＞0.05). The most
significant growth stimulation of myoblasts in culture was achieved by adding 3.0 ng/㎖ of bFGF, producing a stimulation effect up to 2.01-fold. All myoblasts treated with growth factors differentiated into myotube.
Conclusion: Our findings indicate that bFGF and LIF stimulate the proliferation of myoblast, which may result in an effective way in producing large numbers of myoblasts for clinical myoblast transplantation in DMD patients.
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KEYWORD
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